Application Of Zno Nanoparticles In Biological Treatment Of Municipal Wastewater

Abstract

With the fast development of nanomaterials and nanotechnology, environmental nanotechnology has captivated increasing concerns in the past few decades. There are several nanomaterials in the earth, and a variety of modification held in future development. These Zinc nanoparticles illustrate antibacterial, anti-corrosive, and UV filtering premises. In the study, we determine the antibacterial action of nanoparticles of Zinc Oxide for the curing of city wastewater. Sample of wastewater were collected from sewage water, make three samples the sample I exposed to UV, and Sample II is treated with chlorine while other is control. Samples were than cultured on NA and EMB agar, next day microscopy, biochemical test, spot test to identify the specie was performed accordingly. From control sample Escherichia coli while from UV exposed and chlorine treated organism identified as Staphylococcus. epidermidis  respectively, Nano particles of Zinc were prepared by mechano-chemical method then these nanoparticle were characterize by transmission electron microscopy, then their antimicrobial action obtain to separated wastewater bacteria were calculated by determining the disk diffusion test results shows clear zone of inhibition against both Escherichia coli and Staphylococcus epidermidis on MHA, the MIC test was performed and we observed MIC at concentration 32 and 64 concentration in S.epidermidis while in E.coli concentration 64 . Wastewater obtain after chlorination and UV exposure are compared by the previous results. The resultant of experiment suggests that to teat wastewater, ZnO nanoparticles can act as antimicrobial agent. The class of bacterial strains is directly depended by effectiveness of antimicrobial action of nanoparticles of ZnO, in other words activity increase by increase in the concentration of nanoparticles.