Isolation and Study of Cellular Components of Aerobacillus Polymyxa along with Its Comparison in Soil Layers

Abstract

Objectives: The main objective of this research was to isolate and to study the cell morphology and biochemical reactions of Aerobacillus polymyxa and Bacillus megaterium along with its habitat either in deep soil or aerobic soil.

Background: AeroBacillus is from the family of PaneBacillus polymxa and Bacillus megaterium belongs from “Bacillaceae†family. These two organisms are gram positive, non-pathogenic bacteria found in soil that helps in nitrogen fixation. They both are equally important today but the main aim of this research to isolate them from the soil due to the characteristic importance of A. polymyxa to produce antibiotic and helps to remove biofilm formation where as B. megaterium is a good a source of producing industrial proteins due to its larger size than any other organisms.

Methodology: Total16 samples were collected from aerobic & anaerobic soil, water and milk. The soil samples were cultured on TGB media and NA for four days.

Results: Out of 16 samples 9 samples have shown positive results for the colonies of A. polymyxa and out of 16 samples 12 samples showed positive results for B. megaterium further confirmed by biochemical reactions.