Pathogens Identification and Evaluation of Nigella sativa’s (Kalonji) Antibacterial Activity

  • Urooj Arif
Keywords: Clinical samples, Disc diffusion method, Nigella sativa, N. sativa oil, Thymoquinone.


To examine the presence of different pathogens capable of causing diseases in the humans, bacteria
were isolated from clinical samples. 40 different clinical samples were collected from which different
pathogens were isolated and identified by using conventional methods. In this article we also checked
the antibacterial activity of Nigella sativa. The Nigella sativa commonly known as kalonji are use in
herbal medicine, throught the world for the prevention and treatment of many diseases. The kalonji
oil has many properties like antibacterial, antiparasitic, antioxidant etc. kalonji oil was used to study
the antibacterial activity against 5 clinical isolates of bacteria. Disc diffusion technique was applied
on inoculated Muellar Hinton agar plates. Among Gram positive bacteria only Staphylococcus aureus
was used, Among Gram negative bacteria Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus,
and E.coli were used. The oil showed