Green Synthesis of Silver Nanoparticles and Their Effect on the Activity of Alkaline Phosphatase

Authors

  • Asfandyar Khan Anti Sera Lab, National Institute of Health, Islamabad, Pakistan.
  • Aleem Munir Anti Sera Lab, National Institute of Health, Islamabad, Pakistan.
  • Isfahan Tauseef Hazara University Mansehra, KP, Pakistan.

Keywords:

AgNPs, Spectrophotometry, Alkaline Phosphatase (ALP)

Abstract

Green synthesis of silver nanoparticles (AgNPs) is an eco-friendly, cost-effective, and less toxic approach. Silver nitrate is used as a reducing agent and is mixed with the lemon extracts via a top-down approach. AgNPs were synthesized using different parts of lemon extracts including lemon fiber, outer layer of lemon, and lemon seeds. The formation of Ag NPs was confirmed by the change in color from yellow to reddish brown. These AgNPs were then characterized via UV-VIS spectrophotometry and depicted maximum absorption between 420 to 440 nm. An alkaline phosphatase (ALP) assay was performed for these silver nanoparticles at an absorbance of 405 nm. The AgNPs enhanced the enzymatic activity of ALP.

Downloads

Published

2023-12-30

Issue

Vol. 14 No. 2 (2023): Volume 14 (2), December 2023

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.