Isolation of Gram Positive and Gram Negative Organisms from Pus Samples: One Center Study


  • Naheed Afshan Jinnah University for Women, Karachi
  • Madiha Shahid Jinnah University for Women, Karachi


Abscesses, Gram positive, Gram negative, Post surgical infection


The contamination of pus organisms in infected wounds, ears, brain abscesses and post surgical infection is a common problem for many patients which causes great distress in terms of discomfort, delayed healing and significantly increased healthcare cost by creating systemic infection and complication. The aim of the study was to find out the frequency as well as antimicrobial susceptibility of gram positive and gram negative organisms isolated from pus samples. The study was conducted at the Microbiology Department of Jinnah University for Women Karachi, Pakistan during September to November 2012. For this purpose pus samples collected from infected wounds, ears and post surgical infection, from different Health care Hospitals. Out of 35 positive cultures 11(31%) of gram positive followed by Staphylococcus aureus was identified 8(22.85%) and Streptococcus pyogenes 2(5.71%), Streptococcus epidermidis 1(2%) on the basis of colony morphology, gram’s stain, catalase and DNase test. 3(8.57%) were gram negative like Pseudomonas spp 3(8.57), Klebsiella spp 4(11.42%), E.coli 9(25.71%) and Candida spp 1(2.85%) were identified on the basis of microbiological and biochemical test.

Author Biographies

Naheed Afshan, Jinnah University for Women, Karachi

Department of Microbiology

Madiha Shahid, Jinnah University for Women, Karachi

Department of Microbiology