Modification of Process Parameters for Enhanced Lipase Induction from Bacillus SR1
Keywords:Isolation, lipase, Bacillus, fermentation, triacylglycerol
The enzymes catalyze the cleavage of triacylglycerols into fatty acids and glycerols are referred to as lipases (EC 22.214.171.124). Lipases are widely distributed in flora and fauna. Microbial lipases are of great importance than lipases from plants and animals due to their catalytic activity, ease of production and optimization. Lipases have tremendous industrial applications such as in the processing of fats and oils, detergents and degreasing formulations, food processing, the synthesis of fine chemicals, paper manufacture, and production of cosmetics, and pharmaceuticals. Therefore, a potential lipase producing bacterial strain was isolated and identified as gram +ve Bacillus SR1. Among different oils tested, olive oil was found to be the favorable substrate for lipase induction. Additionally, lipase induction was observed highest in 24 hours of fermentation at 37?C and pH 7.5.