Oral Health Status: The Level of Oral Microbial Flora in Healthy Girls

Abstract

The normal oral flora comprises a diverse group of micro-organisms and then 300 species inhabit the
oral cavity of which about 50 are found routinely and account for the majority of the cultivable strains,
these factors, together with the fact that the indigenous microbiota plays an important role in health
and disease of a human and animals.It contribute to the development of the immune system and
provide resistance to colonization by allochthonous or pathogenic micro-organisms. The aim of our
study is to determine the most frequent micro-organisms as normal flora in healthy individuals. A
total of 30 healthy students, all belongs to Jinnah University for Women of same age group were
studied. Throat swabs were plated with human-blood agar, chocolate agar and apply bacitracin disk
on both plates, SDA agar were incubating for 24 hours for isolation of Candida species. Organisms
were cultured on Blood agar, SDA agar, Mannitol salt agar to observe the colonies. Different spot test
like catalase and coagulase test were performed for identification of Staphylococcus species. Identification
was also done by microscopic examination by gram stain. Catalase-positive, gram-positive rods were
found to be as most frequent micro-organisms as normal flora of healthy students and were only
micro-organisms detected 46.6% and total Staphylococcus species were (30%); Staphylococcus aureus
( 13%) and other Staphylococcus species (17 %) where as Streptococcus species (catalase -negative
gram positive cocci) isolated from oral specimen of healthy individuals.