Microbiological Analysis of Ophthalmic Solutions

Authors

  • Sana Siraj Khan Jinnah University for Women, Karachi Pakistan.
  • Amna Shafiq Jinnah University for Women, Karachi Pakistan.
  • Syeda Ghufrana Nadeem Jinnah University for Women, Karachi Pakistan.

Keywords:

contactlenses, Acanthamoeba, hygiene

Abstract

Microbial corneal contamination is the most genuine confusion of contact lens wear. A defiled contact lens arrangement and embellishments can go about as a supply for microorganisms that could conceivably agree contact lens wear and prompt sight debilitating unfavorable occasions. The rate of lens case arrangements ruining is normally more than half. The point of our exploration study to detach microorganisms from contact lens stockpiling arrangements and to describe and recognized by front line morphology, common characteristics and biochemical test. We take distinctive specimens of lens consideration stockpiling arrangement from asymptomatic patients. Culture tests on blood agar and macconkey agar for gram positive and negative microscopic organisms, SDA for contagious separation and PAS (Periodic acid– Schiff ) for Acanthamoeba segregation. Furthermore, we discovered 100% lens care stockpiling arrangements debased from microorganisms. In which our outcome demonstrates that half specimens were defiled with gram positive microorganisms while half examples were tainted with gram negative microscopic organisms, and 67% Fungus were available. Acanthamoeba were not disengaged in any tried specimen, might be on the grounds that it once in a while separated invitro from tests. Subsequently it is reduced that it is critical to keep up abnormal state of contact lens cleanliness and that you tail all the cleaning systems. Genuine consideration ought to be taken by contact lens clients, to keep up abnormal state of cleanliness, appropriate changing of lens consideration arrangement after some time.

Author Biographies

Sana Siraj Khan, Jinnah University for Women, Karachi Pakistan.

Department Of Microbiology

Amna Shafiq, Jinnah University for Women, Karachi Pakistan.

Department Of Microbiology

Syeda Ghufrana Nadeem, Jinnah University for Women, Karachi Pakistan.

Department Of Microbiology

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Published

2015-08-08