Antibacterial Activity of Camellia sinensis Extract on Uropathogenic, Extended Spectrum Beta Lactamase Producing E.coli

Abstract

The potential of antibacterial activity of Camellia sinensis extract was studied on uropathogenic Extended Spectrum Beta Lactamase (ESBL) producing E.coli has become the major cause of all the hospital acquired infections resulting in about 80-85% of all Urinary Tract Infections. The Multi drug resistant organisms are now difficult to treat with beta lactam antibiotics, which were used early to treat infections caused by this uropathogen. In order to avoid resistance with antibiotics we need to look for alternative treatment methods one such as; the evaluation of the antibacterial activity of Green Tea Extract which is polyphenol in nature. It contains catechin, epicatechin (EC), epigallocatechin-3-gallate (EGCg), epigallocatechin (EGC), epicatechin-3-gallate (ECG) which is responsible for greatest antimicrobial activity. Green tea is also considered as a protective agent against many types of cancers. The components exists in green tea are antioxidant and antimutagenic. The antibacterial activity of Camellia sinensis (Green tea) extract was tested on 10 strains of E.coli. The concentrations used were 110mg/ ml, 130mg/ml, 150mg/ml, 170mg/ml, and 190mg/ml on agar well diffusion assay. All strains give large zones of inhibition ranging from 9-25 millimeter in diameter. The MIC of all strains ranged in between 110 to 170mg/ml, most of the strains showed MIC at 110mg/ml and at 130 mg/ml. (30%) was found susceptible at 130mg/ml, (50%) at 130mg/ml, while (10%) was found susceptible at 150mg/ml and 170mg/ml. The emergence of ESBL has been wide in centers of every different country and has become a common problem which should be resolve soon.